venteon ultra used in two-photon microscopy
In a recent article published in the July edition of the European Biopharmaceutical Review on two-photon microscopy, Laser Quantum talks about the use of the venteon ultra few-cycle laser as an excitation source that offers an ultra-broad spectral output and different methods for GVD pre-compensation. The venteon ultra delivers >240 mW of sub-5.5 fs short laser pulses directly out the box (>3 nJ @ 80 MHz repetition rate). It has the shortest available pulse duration and is ideal as an excitation laser for two-photon microscopy, especially for highly efficient excitation and covering a wide range of fluorophores without the need for tuning. Short pulses have been shown to increase excitation efficiency, higher contrast, deeper images, lower photo-damage and the simultaneous excitation of multiple dyes without the need for tuning.
The article discusses the requirement for pulse broadening compensation and the different approaches available. With these easy to use alternatives for GVD compensation, a microscopist can achieve all the reported benefits of the ultra-short pulse (broad spectrum) laser without the need for expertise in photonics.
To read the article please visit the EBR website or download the article here.
The article is taken from European Biopharmaceutical Review July 2016, pages 58-61. © Samedan Ltd