March 23rd, 2017 | Company News

Two-photon non-linear microscopy

The venteon ultra has recently been used in two-photon non-linear microscopyTwo-photon non-linear microscopy is a flourescence technique that enables the imaging of living biological samples, especially useful in neuroscience, where obtaining high resolution and precise results is always a challenge. The extreme bandwidth of the venteon ultra ensures ultra-short pulse durations that result in high photon flux at the focal point of the laser, thus increasing the fluorescent signal and image quality. The venteon ultra has been instrumental in the research reported in the two following papers to achieve precise results in two-photon microscopy:

Isobe – Multifarious control of two-photon excitation of multiple fluorophores achieved by phase modulation of ultra-broadband laser pulses – Opt Extr

Hashimoto – Measurement of two-photon excitation spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy – Appl Opt

Another application that benefits from the ultrabroadband spectrum of the venteon ultra is CARS / SERS, a spectroscopic method sensitive to vibrational signatures of molecules similar to Raman spectroscopy. The octave spanning spectrum is an ideal laser source for any CARS / SRS applications and this has been shown in the following paper:

Isobe – Single-pulse coherent anti-Stokes Raman scattering microscopy employing an octave spanning pulse – Opt Extr

The venteon ultra has unique spectral properties, it delivers >240 mW of sub-5.5 fs laser pulses directly out the box. It has a pulse energy >3 nj @ 80 MHz repetition rate, making it ideal for numerous applications.

To find out more about the exceptionally short pulse duration of the venteon ultra and how it can benefit you, please contact us.