April 21st, 2016 | Company News

Noncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopy

Recent studies by G. Scarcelli et al. detail how our torus 532 is being used to develop a label free microscopy technique based on Brillouin scattering, capable of imaging intracellular processes with vastly improved optical resolution. Intracellular processes such as the interaction between the liquid and solid phases in the cytoplasm have a critical role in determining how cells deform and move. The most effective way to monitor these processes is in their natural microenvironments, which requires all measurements to be done in situ, hence the need for a label free mapping technique. This approach will enable further understanding of how cells change during ageing, injury healing and disease pathogenesis.
Our torus 532 was used as the light source for the Brillouin confocal microscope. The torus is a frequency-doubled Nd-YAG laser emitting a single longitudinal mode at 532nm, with powers ranging between 50mW-750mW, and is ideal for Brillouin microscopy due to the TruLoQ™ active locking mechanism; the torus continually tracks its longitudinal mode efficiency and adjusts the cavity length accordingly, minimizing frequency drift and eliminating mode-hop. It has a unique “virtual ring cavity” design that enables specifications typical of a physical ring cavity, but with the added benefits of high ruggedness and low sensitivity to shock, vibration and background temperature changes.
Please see the 2015 Nature Methods reference and abstract for further experimental details.