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April 15th, 2016 | Company News

Measurement of two-photon excitation spectra of fluorescence proteins with nonlinear Fourier-transform spectroscopy

Work published by Hashimoto et al., from Riken Advanced Science Institute, Japan, makes use of our venteon ultra laser (formally Known as Venteon OS) from Laser Quantum as part of their work into ‘Measurement of two-photon excitation spectra of fluorescence proteins with nonlinear Fourier-transform spectroscopy.’

Biological imaging of live cells is an important part of microscopy and developing techniques such as two-photon excitation fluorescence to achieve high spatial resolution, 3-Dimensional imaging, and the reduction of photobleaching is crucial. Ultrafast laser technology has played a key part in advancing this technique with the unique properties of ultrashort femtosecond pulses. A nonlinear Fourier-transform spectroscopy technique was used to obtain spectroscopic information to further the investigation. This is a reliable technique in this experiment, as the broadband spectroscopic information is not significantly influenced by temporal fluctuations in the detected signal.

Our venteon ultra was used as the Ti:Sapphire mode-locked laser, operating at 80MHz for the ultra-broadband light source. The venteon ultra is a robust source for ultrashort pulses <5fs on a compact footprint; its monoblock design has an average power of >240mW and a pulse energy of >3nJ with a spectral bandwidth of >380nm @ -10dBc.

The full paper reference is:

Hiroshi Hashimoto, Keisuke Isobe, Akira Suda, Fumihiko Kannari, Hiroyuki Kawano, Hideaki Mizuno, Atsushi Miyawaki, and Katsumi Midorikawa, “Measurement of two-photon excitation spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy,” Appl. Opt. 49, 3323-3329 (2010).



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