Raman spectroscopy and microscopy are techniques used to study vibrational, rotational and other low frequency modes in a molecular system. Laser light interacts with the system resulting in the energy of the laser photons being shifted up or down. The shift in energy gives information about the molecular bonds and hence the molecule itself. This can be used to identify the molecule being tested.
The laser excitation needs to be in the wavelength range 400-800nm and the power required depends largely on the analysis that is being performed and the area of sample being excited. 532, 473 and 660nm are commonly used wavelengths. The bandwidth of the excitation beam can be a limiting factor in the resolution of the Raman system with lower bandwidths producing higher resolution. Single longitudinal mode lasers provide the highest resolution, but can introduce variation if they are not locked to that single mode.
Ruggedness and stability of the system are also factors to be considered when selecting the right laser for any application. Many of our lasers are suited perfectly to the needs of Raman spectroscopy and microscopy.
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